Keywords: Broilers, diet formulation, dose-response, lysine, performance. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP VCentro de Aquicultura da Universidade Estadual Paulista, Via de Acesso Prof. IVBOAV Alimentos LTDA, Estrada Vicinal Casa Branca/Tambaú, km 8,5, CEP 13700-000, Casa Branca, SP IIIDepartamento de Zootecnia, Universidade Federal do Piauí, BR 135, km 03, CEP 64900-000, Bom Jesus, PI IIDepartamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Via de Acesso Prof. ICentro de Ciências Agrárias e Ambientais, Universidade Federal do Maranhão, BR 222, km 04, CEP 65500-000, Chapadinha, MA Siqueira JC I, II Sakomura NK II Dourado LRB III Ezequiel JMB II Barbosa NAA IV Fernandes JBK V Lysine levels required for optimal feed conversion ratio of broilers during the pre-starter and starter phases were estimated at 1.361 and 1.187%, which are equivalent to lysine intake of 0.340 and 0.797 g/day, respectively.īroilers diet formulation dose-response lysine performanceĭiet formulation techniques and lysine requirements of 1- to 22-day-old broilers The results suggest that the dilution technique favored the performance potential and better met lysine requirements compared with the supplementation technique. For the diluted diet, a diet high in crude protein (CP) and relatively low in lysine was formulated and to which was added a protein-free diet until lysine levels were similar to those described above for the supplemented diet.

For the supplemented diet, a basal diet was formulated to meet the nutritional requirements, then L-lysine HCl was added to achieve digestible lysine levels of 0.975, 1.082, 1.189, 1.296 and 1.403% in the pre-starter diets and 0.840, 0.932, 1.024, 1.116 and 1.208% in the starter diets.

In each experiment, 1,200 male Cobb 500 chickens were randomly distributed according to a 5x2 factorial arrangement (lysine level x formulation technique) with six replicates of 20 birds each. Two experiments were carried out to compare two techniques (amino acid supplementation and dilution) for formulating experimental diets for pre-starter (1 to 8 days) and starter (8 to 22 days) broiler chicks and to estimate digestible lysine requirements using the dose-response method.

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